5 edition of Molecular cloning of hormone genes found in the catalog.
Includes bibliographies and index.
|Statement||edited by Joel F. Habener.|
|Series||Molecular biology and biophysics|
|Contributions||Habener, Joel F.|
|LC Classifications||QH442.2 .M65 1987|
|The Physical Object|
|Pagination||xvii, 441 p. :|
|Number of Pages||441|
|LC Control Number||86019980|
Plasmids have been repurposed and engineered as vectors for molecular cloning and the large-scale production of important reagents such as insulin and human growth hormone. An important feature of plasmid vectors is the ease with which a foreign DNA fragment can be introduced via the multiple cloning site (MCS). A rhesus monkey genomic library was screened with a cDNA for the glycoprotein hormone α-subunit. Genomic clones hybridizing with exon-specific probes were selected and the DNA sequences were determined for kb of 5′-flanking DNA, all four exons, the second and third introns, all exon–intron junctions, and bp of 3′-flanking by:
Despite the importance of WRKY genes in plant physiological processes, little is known about their roles in Panax ginseng C.A. Meyer. Forty-eight unigenes on this species were previously reported as WRKY transcripts using the next-generation sequencing (NGS) technology. Subsequently, one gene that encodes PgWRKY1 protein belonging to subgroup II-d was cloned and functionally by: The Polymerase Chain Reaction You can isolate virtually any DNA sequence by means of the polymerase chain reaction, or PCR. PCR uses repetitive cycles of primer‐dependent polymerization to amplify a given DNA.
Genetics and Molecular Research 10 (3): () ©FUNPEC-RP Molecular cloning and sequence analysis of follicle-stimulating hormone beta polypeptide. Lipid‐soluble hormones act usually by gene activation/deactivation. Examples of these hormones include steroids, thyroid hormone, and vitamin A (retinoic acid). The hormones are transported through the circulation in association with a hormone‐binding protein and are soluble in .
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The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune Brand: Humana Press. The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune systems, as well as in embry onic deve.
Molecular Cloning of the Genes Encoding the?-Subunit of Glycoprotein Hormones.- Molecular Cloning of cDNA Encoding the?-Subunit of Human CG.- Molecular Cloning of the Genes Encoding the Human CG?-LH.
Subunits.- Molecular Molecular cloning of hormone genes book of cDNA Encoding the Rat LH?-Subunit.- Molecular Cloning of the Gene Encoding the Rat LH. Molecular cloning of hormone genes. [Joel F Habener;] -- The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune systems, as well as in embry onic.
Molecular Cloning: A Laboratory Manual (Fourth Edition): Three-volume chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis.
They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be /5(17). Molecular cloning: A laboratory manual Medical books Molecular cloning. Medical books Molecular Cloning of Hormone Genes.
Categories: Molecular cloning, Peptide hormones, Molecular clonin. Contributors: Joel F. Habener - Author. Format: Hardcover Download link for Molecular Cloning: A Laboratory Manual. The key principles of molecular cloning were discovered a little over 50 years ago.
Since then, molecular cloning has become one of the most powerful tools of the molecular biology laboratory enabling the expression of the smallest genes, as well as. Description. Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years.
No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and.
Gonadotropin-releasing hormone (GnRH) is a key neuropeptide regulating reproduction in vertebrates. In the present study, we have cloned and identified the cDNA sequence of the prepro-GnRH (rp-GnRH) in the Manila clam Ruditapes open reading frame encodes a protein of 94 amino acids, which consists of a signal peptide, a GnRH dodecapeptide, a cleavage site, and a GnRH Cited by: 6.
Molecular Cloning: A Laboratory Manual (Fourth Edition): Three-volume set Michael R. Green. out of 5 stars Paperback. $ Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) Joe Sambrook. out of 5 stars Paperback. 28 offers from $ s: 2. PCR thereby makes possible the “cell-free molecular cloning” of a DNA fragment in a few hours, compared with the several days required for standard cloning procedures.
This technique is now used routinely to clone DNA from genes of interest directly—starting either from genomic DNA or from mRNA isolated from cells (Figure ).Cited by: 5.
Abstract. Parathyroid hormone (PTH), a peptide of 84 amino acids, is the major hormonal regulator of the level of ionized calcium in extracellular fluid (1).Parathyroid glands have been found in amphibians, reptiles, birds, and mammals, but not in fish or lower vertebrates.
Cloning and expression of human growth hormone in E. coli system. Arab Journal of Biotechnology. 18 (1): hormone by cloning hGH gene into a n expression Molecular Cloning, a. Molecular cloning is an essential technique to create DNA-based experimental tools for expression in bacterial or mammalian cells.
Examples of such DNA constructs include a promoter element fused to a reporter gene or a cDNA sequence under the control of a ubiquitous promoter.
INTRODUCTION. Thyroid hormones (thyroxine and triiodothyronine (T 3)) 2 play a vital role in fetal development and throughout life in humans. T 3, an active form of the thyroid hormones, exerts its effect through binding to its nuclear receptor and regulating expression of targetidentification of T 3-responsive genes in various tissues is important to elucidate T 3 action at Cited by: During the past ten years, refinements in the techniques of recombinant DNA technology have resulted in the cloning of genes encoding approximately 50 different hormonal and regulatory peptides, including those in which the peptides themselves and the mRNAs encoding the peptides are present in only trace amounts in the tissues of origin.
Two of the objectives in the assembly of this book are to pre sent, in one volume, the known primary structures of the genes encoding several of the polypeptide hormones and related regulatory peptides, and to provide an account of the various ap proaches that have been used to identify and select the cloned genes encoding these polypeptides.
Molecular cloning. From Wikipedia, the free encyclopedia. Jump to navigation Jump to search. Diagram of molecular cloning using bacteria and plasmids. Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.
The encoding of multiple peptides in a single mono- cistronic mRNA appears to be a genetic mechanism for the gener- ation of biologic diversification without requiring amplification of gene of the objectives in the assembly of this book are to pre- sent, in one volume, the known primary structures of the genes encoding several of the polypeptide hormones and related.
The pituitary hormone thyrotropin, or thyroid-stimulating hormone (TSH), is the main physiological agent that regulates the thyroid gland. The thyrotropin receptor (TSHR) was cloned by selective amplification with the polymerase chain reaction of DNA segments presenting sequence similarity with genes for G protein-coupled receptors.
Out of 11 new putative receptor clones obtained Cited by:. By cloning the human insulin gene and expressing it in E.
coli, large quantities of insulin identical to the human hormone could be produced in fermenters, safely and efficiently. Production of recombinant insulin also allows specialized variants of the protein to be produced: for example, by changing a few amino acids, longer-acting forms of.Thanks to molecular cloning, reporter genes can be incorporated into DNA plasmids, a common reporter gene is green fluorescent protein or GFP, which emits a green fluorescence when exposed to UV light.
A reporter gene can also be inserted into an alphavirus to show infection in mosquitoes and transmissibility in cells.Chinese kale is a native vegetable in Southern China and the flowering stalk is the most commonly used edible part due to its high glucosinolate content and other nutritional qualities.
The GTR protein played important roles in the glucosinolate transport process. In this study, three BocGTR1 genes were cloned from Chinese kale for the first time. Their gene structure, physicochemical.